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Footage of live Toxoplasma gondii parasite


Paweł Krzyściak1, Dominika Salamon2, Anna Malska3, Barbara Papir4



Introduction

Toxoplasma gondii is an important protozoan causing infections worldwide. It is of major importance in pregnant ladies with severe implications to the foetus, including congenital toxoplasmosis, as well as persons who are immunocompromised, causing life-threatening central nervous system and ocular toxoplasmosis. Diagnostics of the parasite is nowadays mostly based on enzyme-linked immunosorbent assay (ELISA) for different antibody classes, especially IgM, IgG and IgA with some new approaches, too [1].

Novel treatment regimens may be found in the latest edition of the Sanford Guide to Antimicrobial Therapy [2].


Images

The following footage was made during the preparation steps of the Sabin-Feldman Dye Test, which used to be the reference test for toxoplasmosis at our department (Figures 1-2).

The entire film may be seen on the official WJOMI YouTube channel by clicking the link: https://www.youtube.com/channel/
UCv78AAmi2wvjdg1HVHwIXog



figure1

Figure 1. Toxoplasma gondii trophozoites as seen in the Sabin-Feldman Dye Test.

















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figure2

Figure 2. Live T. gondii trophozoites – screenshot of the film: https://youtu.be/h8CVDoRP37w


References

[1] Pietrzyk A, Kochan P, Papir B, Bulanda M. Is there an easier way to differentiate phases of Toxoplasma gondii infection in pregnant women? 26th ECCMID, Amsterdam, The Netherlands, 9-12 April 2016.
[2] Sanford Guide to Antimicrobial Therapy 2017. Gilbert DN, Chambers HF, Eliopoulos GM et al. (eds.) Antimicrobial Therapy Inc., Sperryville 2017.

Conflict of interest: none declared

Authors’ affiliations:
1 Head of the Mycology Department, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland
2 Department of Molecular Medical Microbiology, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland
3 Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland
4 Department of Bacteriology, Microbial Ecology and Parasitology, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland

Corresponding author:
Dr Paweł Krzyściak, Ph.D.
Head of the Mycology Department
Chair of Microbiology
ul. Czysta 18
31-121 Cracow, Poland
e-mail: pawel.krzysciak@uj.edu.pl

To cite this article: Krzyściak P, Salamon D, Malska A, Papir B. Footage of live Toxoplasma gondii parasite. World J Med Images Videos Cases 2018; 4:e16-7.

Submitted for publication: 13 February 2018
Accepted for publication: 20 February 2018
Published on: 1 March 2018



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